Supplemental Materials & Methods:
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چکیده
SUPPLEMENTAL MATERIALS & METHODS: TABLE OF CONTENTS I. PRIMARY DATA GENERATION .........................................................................................3 Cell Culture and Cell Lines Assayed .....................................................................................................................................3 Sub-cellular Fractionation and RNA Isolation (CSHL).......................................................................................................3 CSHL Long (>200) RNA-seq Library Preparation .............................................................................................................4 CalTech Long RNA-seq Library Preparation ....................................................................................................................4 Small (<200) RNA-seq Library Preparation .......................................................................................................................5 CAGE Library Preparation ...................................................................................................................................................6 RNA-PET Library Preparation .............................................................................................................................................8 Splice Junction Validation By RT-PCR and 454 Sequencing ............................................................................................9
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................................................................................22 INTRODUCTION.........................................................................24 RESULTS...................................................................................27 DISCUSSION..............................................................................34 MATERIALS AND METHODS........................
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